CRISPER CAS Classroom Challenge
by Melanie Mundell
Biotechnology I, II, III Teacher, Newark Charter Junior Senior High School
OPTION 1: Out of the Blue CRISPR Use CRISPR-CAS9 to edit the lacZ gene of E. coli bacteria and determine success via blue white screening OPTION 2: CRISPR In A Box Use CRISPR-CAS12a to edit the lax Z gene of E. coli bacteria and determine the success via blue white screening |
Project Resources:
- Project Framework
- Subject(s): Biotechnology
- Learning Outcomes:
- Students will be able to:
- Describe CRISPR CAS-9 techniques for gene editing. Other CAS proteins can be used.
- Describe all the major components of CRISPR CAS editing: CRISPR, CAS proteins, CAS-9, guide RNA, PAM, etc
- Evaluate the success of an applied CRISPR experiment.
- Research future applications of CRISPR-CAS-9
- CRISPR Kits Researched
- Students will be able to: