CRISPER CAS Classroom Challenge

by Melanie Mundell

Biotechnology I, II, III Teacher, Newark Charter Junior Senior High School

OPTION 1: Out of the Blue CRISPR
Use CRISPR-CAS9 to edit the lacZ gene of E. coli bacteria and determine success via blue white screening

OPTION 2: CRISPR In A Box
Use CRISPR-CAS12a to edit the lax Z gene of E. coli bacteria and determine the success via blue white screening

Project Resources:

  • Project Framework
  • Subject(s): Biotechnology
  • Learning Outcomes:
    • Students will be able to:
      1. Describe CRISPR CAS-9 techniques for gene editing. Other CAS proteins can be used.
      2. Describe all the major components of CRISPR CAS editing: CRISPR, CAS proteins, CAS-9, guide RNA, PAM, etc
      3. Evaluate the success of an applied CRISPR experiment.
      4. Research future applications of CRISPR-CAS-9
    • CRISPR Kits Researched

 

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